Optimizing AAV Production: Accelerating Discovery of Small Molecule Enhancers using High Throughput Virology and Design of Experiments

This poster highlights Virica’s accelerated discovery of small molecule enhancers for AAV production by combining High-Throughput Virology (HTV™) with Design of Experiments (DoE). Using HTV, more than 1,000 molecules were rapidly screened, uncovering compounds that boosted AAV yields by up to 2.5-fold in genomic titer and 6-fold in functional titer at bioreactor scale. The integration of DoE streamlined simultaneous testing of multiple variables, significantly speeding up process optimization. Together, these methods demonstrate the effectiveness of Virica’s VSE™ technology in enhancing viral vector manufacturing efficiency.