Increased Viral Vector Yield: Development and Scale-Up Validation of Small Molecule Enhancers

Poster showcases the development and scale-up validation of Virica’s Viral Sensitizer Enhancers (VSEs™), proprietary small molecules that temporarily reduce cellular defenses to increase viral vector yield and quality. Through high-throughput screening and scale-up studies, VSEs demonstrated up to 2.2-fold increases in AAV8 production across small and 5L bioreactor scales. VSEs also showed significant improvements in lentiviral titers in an inducible producer cell line at shake flask scale. Importantly, these enhancers are metabolized and cleared during processing, leaving no detectable residues. Virica also presents a case study illustrating how VSEs can reduce AAV manufacturing costs by enabling higher productivity in smaller bioreactors, offering both performance and economic advantages.