Increase Viral Vector Yield: Small Molecule Enhancers Validation at Multiple Scales

This poster highlights the validation of Virica’s Viral Sensitizer Enhancers (VSE™), proprietary small molecules that increase viral vector yield and quality by suppressing cellular antiviral defenses. The data demonstrates successful VSE-driven benefits at multiple scales, including shake flasks, Ambr® 15 systems, and 5L bioreactors, with a 2.2-fold increase in AAV8 yield. VSEs also showed a significant 10x enhancement of MVA production across diverse strains and at bioreactor scale. Easily added to media during transfection/infection, VSEs are rapidly metabolized and removed via standard purification, as confirmed by sensitive HPLC and MS assays. These findings support the scalability and safety of VSEs in upstream viral manufacturing processes.