{"id":3613,"date":"2025-04-20T13:17:05","date_gmt":"2025-04-20T17:17:05","guid":{"rendered":"https:\/\/www.viricabiotech.com\/staging\/?post_type=avada_portfolio&#038;p=3613"},"modified":"2025-06-19T14:39:56","modified_gmt":"2025-06-19T18:39:56","slug":"pfizer-paper-production-of-raav-by-plasmid-transfection-induces-antiviral-inflammatory-responses-in-suspension-hek293-cells","status":"publish","type":"avada_portfolio","link":"https:\/\/www.viricabiotech.com\/staging\/resources\/pfizer-paper-production-of-raav-by-plasmid-transfection-induces-antiviral-inflammatory-responses-in-suspension-hek293-cells\/","title":{"rendered":"Pfizer Paper \u2013 Production of rAAV by plasmid transfection induces Antiviral inflammatory responses in suspension HEK293 cells"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:0px;--awb-padding-right:0px;--awb-padding-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-1\"><p>Innate cellular defenses are a persistent barrier to viral vector manufacturing. The article shows that innate cellular defenses, triggered by plasmid transfection during rAAV production in HEK293 cells, cause antiviral and inflammatory responses. This innate response, including activation of interferon-stimulated genes and pathogen recognition receptors, limits vector yield and highlights opportunities for improving production efficiency by managing these cellular pathways\u200b<\/p>\n<\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2605,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","meta":{"_acf_changed":false,"content-type":"","footnotes":""},"portfolio_category":[82],"portfolio_skills":[],"portfolio_tags":[70],"class_list":["post-3613","avada_portfolio","type-avada_portfolio","status-publish","format-standard","has-post-thumbnail","hentry","portfolio_category-article","portfolio_tags-cell-gene-therapies"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/avada_portfolio\/3613","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/avada_portfolio"}],"about":[{"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/types\/avada_portfolio"}],"author":[{"embeddable":true,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/comments?post=3613"}],"version-history":[{"count":1,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/avada_portfolio\/3613\/revisions"}],"predecessor-version":[{"id":3614,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/avada_portfolio\/3613\/revisions\/3614"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/media\/2605"}],"wp:attachment":[{"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/media?parent=3613"}],"wp:term":[{"taxonomy":"portfolio_category","embeddable":true,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/portfolio_category?post=3613"},{"taxonomy":"portfolio_skills","embeddable":true,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/portfolio_skills?post=3613"},{"taxonomy":"portfolio_tags","embeddable":true,"href":"https:\/\/www.viricabiotech.com\/staging\/wp-json\/wp\/v2\/portfolio_tags?post=3613"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}